5HJI

Crystal Structure of Pyrococcus abyssi Trm5a complexed with adenosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-05
Detector	OXFORD ONYX CCD
Wavelength(s)	1.5
Spacegroup name	P 21 21 21
Unit cell lengths	52.727, 54.718, 128.948
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.574 - 2.200
R-factor	0.1954
Rwork	0.193
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hjk
RMSD bond length	0.003
RMSD bond angle	0.809
Data reduction software	CrysalisPro
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.574	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.129	0.550
Number of reflections	19382
<I/σ(I)>	8	2.3
Completeness [%]	99.2	98.8
Redundancy	3.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	10-15% PEG3350, 100mM HEPES, pH7.5, 100mM Ca(OAc)2 and 100mM KCl