5HJ0
Crystal Structure of Mis18 'Yippee-like' Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.2825 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 121.069, 121.069, 73.211 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.630 - 2.640 |
R-factor | 0.22176 |
Rwork | 0.220 |
R-free | 0.26030 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.753 |
Data reduction software | xia2 |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 4.000 |
High resolution limit [Å] | 3.900 | 3.900 |
Rmerge | 0.095 | 0.103 |
Number of reflections | 5669 | |
<I/σ(I)> | 15.3 | 3.7 |
Completeness [%] | 97.2 | 93.9 |
Redundancy | 9.9 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | Diffraction quality crystals were obtained using well buffer containing 0.2 M Ammonium chloride/formate/acetate/phosphate and 20% PEG 3350 (with the measured pH of the solution in the range of 6.2 to 8). 15-20 mg/ml protein sample was mixed with the well buffer in a 1:1 ratio. | |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | Diffraction quality crystals were obtained using well buffer containing 0.2 M Ammonium chloride/formate/acetate/phosphate and 20% PEG 3350 (with the measured pH of the solution in the range of 6.2 to 8). 15-20 mg/ml protein sample was mixed with the well buffer in a 1:1 ratio. |