5HHZ
Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (ZmCKO4) in complex with 6-(3-methylpyrrol-1-yl)-9H-purine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.066, 80.066, 203.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.1977 |
| Rwork | 0.197 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o95 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS (Jun 17, 2015) |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.460 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Total number of observations | 326446 | |
| Number of reflections | 44421 | |
| <I/σ(I)> | 22.58 | 2.94 |
| Completeness [%] | 97.1 | 97.1 |
| Redundancy | 7.35 | 7.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES pH 7,5, 50% MPD, infiltrated for 1h with 2 mM 6-(3-methylpyrrol-1-yl)-9H-purine (MPP) in DMSO and 5% glycerol |
