5HHX
Inhibiting complex IL-17A and IL-17RA interactions with a linear peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 113.400, 113.400, 86.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.230 - 3.000 |
| R-factor | 0.1802 |
| Rwork | 0.176 |
| R-free | 0.26340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vxs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.230 | 3.350 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.134 | 1.150 |
| Number of reflections | 13244 | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.5 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M NaOAc, pH=4.5 and 30% PEG MME 5000 |
