5HHV
Inhibiting complex IL-17A and IL-17RA interactions with a linear peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 110.200, 110.200, 90.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.430 - 2.200 |
| R-factor | 0.1999 |
| Rwork | 0.198 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vxs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.430 | 2.460 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.095 | 0.149 |
| Number of reflections | 32509 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 8.2 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | .02 mM CdCl2, 0.02 M MgCl2, 0.02 M NiCl2, 0.1 M NaOAc pH=4.2-4.9, and 24-28 % PEG MME 2000 |
