5HHJ
Reverse transcriptase domain of group II intron maturase from Roseburia intestinalis in P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.080, 88.060, 79.760 |
| Unit cell angles | 90.00, 95.44, 90.00 |
Refinement procedure
| Resolution | 44.030 - 1.200 |
| R-factor | 0.1242 |
| Rwork | 0.123 |
| R-free | 0.14750 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5hhk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.122 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2254: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.030 |
| High resolution limit [Å] | 1.200 |
| Number of reflections | 178024 |
| <I/σ(I)> | 16.04 |
| Completeness [%] | 99.0 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291.15 | 100 mM SPG pH 9.0, 23% PEG 1500 |
