5HHE
Human Beclin 1 coiled-coil domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.200, 71.575, 58.446 |
| Unit cell angles | 90.00, 112.55, 90.00 |
Refinement procedure
| Resolution | 40.150 - 1.460 |
| R-factor | 0.1733 |
| Rwork | 0.172 |
| R-free | 0.20810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q8t |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.704 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.150 | 1.570 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Number of reflections | 38596 | |
| <I/σ(I)> | 15.91 | 2.02 |
| Completeness [%] | 99.7 | 97.9 |
| Redundancy | 7.5 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 293 | 39% 2-methyl-2, 4-pentadiol, 9% PEG400 and 100 mM Tris buffer, pH 7.5. |
