5HH0
Crystal structure of human Naa60 in complex with CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.100, 73.437, 43.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.550 - 1.600 |
| R-factor | 0.1814 |
| Rwork | 0.181 |
| R-free | 0.18390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hgz |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.651 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.021 | 0.325 |
| Number of reflections | 28597 | |
| <I/σ(I)> | 31.8 | 2 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 6.9 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7.5 | 293.15 | 0.2 M L-Proline, 0.1 M HEPES pH7.5, 10% w/v PEG 3350 |
