5HH0
Crystal structure of human Naa60 in complex with CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 67.100, 73.437, 43.165 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.550 - 1.600 |
R-factor | 0.1814 |
Rwork | 0.181 |
R-free | 0.18390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hgz |
RMSD bond length | 0.017 |
RMSD bond angle | 1.651 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.021 | 0.325 |
Number of reflections | 28597 | |
<I/σ(I)> | 31.8 | 2 |
Completeness [%] | 99.6 | 98.5 |
Redundancy | 6.9 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 7.5 | 293.15 | 0.2 M L-Proline, 0.1 M HEPES pH7.5, 10% w/v PEG 3350 |