5HGR
Structure of Anabaena (Nostoc) sp. PCC 7120 Orange Carotenoid Protein binding canthaxanthin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.989, 53.371, 65.043 |
| Unit cell angles | 93.23, 112.93, 93.09 |
Refinement procedure
| Resolution | 38.116 - 1.681 |
| R-factor | 0.1709 |
| Rwork | 0.170 |
| R-free | 0.20170 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.835 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.116 | 1.770 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.105 | 0.565 |
| Number of reflections | 70766 | |
| <I/σ(I)> | 8.1 | 2.5 |
| Completeness [%] | 95.1 | 86.1 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM sodium formate, pH 6, 10% PEG 3350 |
