5HGN

Hexameric HIV-1 CA, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 6
Unit cell lengths	90.730, 90.730, 56.751
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.690 - 1.900
R-factor	0.2013
Rwork	0.200
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h4e
RMSD bond length	0.007
RMSD bond angle	0.998
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.4.2)
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.690	26.690	1.940
High resolution limit [Å]	1.900	8.910	1.900
Rmerge	0.121	0.074	0.748
Rpim	0.059	0.035	0.379
Total number of observations	106747	1165	6748
Number of reflections	21070
<I/σ(I)>	8.3	17.1	2.1
Completeness [%]	99.8	96.9	100
Redundancy	5.1	5.4	4.8
CC(1/2)	0.992	0.994	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	PEG550MME (13-14%), KSCN (0.15M), TRIS (0.1M, pH 8.5)