5HGL

Hexameric HIV-1 CA, open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-03-27
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	89.690, 159.270, 249.399
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 3.100
R-factor	0.2517
Rwork	0.250
R-free	0.28190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h4e
RMSD bond length	0.008
RMSD bond angle	1.166
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.14)
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.990	19.990	3.270
High resolution limit [Å]	3.100	9.800	3.100
Rmerge	0.094	0.069	0.552
Rpim	0.065	0.048	0.411
Total number of observations	89050	2681	10474
Number of reflections	31869
<I/σ(I)>	7.1	13.9	1.9
Completeness [%]	97.4	84.6	92
Redundancy	2.8	2.9	2.4
CC(1/2)	0.986	0.976	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	PEG550MME (13-14%), KSCN (0.15M), TRIS (0.1M, pH 8.5)