5HGE
Filamentous Assembly of Green Fluorescent Protein Supported by a C-terminal fusion of 18-residues, viewed in space group P212121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.160, 62.910, 69.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.570 - 1.863 |
R-factor | 0.1731 |
Rwork | 0.169 |
R-free | 0.21140 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4piq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.130 |
Data scaling software | Aimless (0.5.17) |
Phasing software | BUSTER |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.570 | 1.869 |
High resolution limit [Å] | 1.863 | 1.863 |
Rmerge | 0.106 | 0.557 |
Rpim | 0.060 | 0.317 |
Total number of observations | 69078 | 10135 |
Number of reflections | 18522 | |
<I/σ(I)> | 9.4 | 2 |
Completeness [%] | 96.0 | 96.6 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.997 | 0.836 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 30% MPD, 20% ethanol, 20 mM HEPES, pH 7.5, 10 mM NaCl |