5HE3
Bovine GRK2 in complex with Gbetagamma subunits and CCG224411
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.031, 239.412, 208.625 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.740 |
R-factor | 0.1981 |
Rwork | 0.195 |
R-free | 0.25710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.567 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.560 | 2.800 |
Rmerge | 0.104 | 0.051 | 0.936 |
Rmeas | 0.114 | 0.057 | |
Rpim | 0.053 | 0.026 | 0.486 |
Total number of observations | 168944 | ||
Number of reflections | 36395 | ||
<I/σ(I)> | 8.1 | ||
Completeness [%] | 93.3 | 96.8 | 93.5 |
Redundancy | 4.6 | 4.3 | 4.5 |
CC(1/2) | 0.996 | 0.769 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 50 mM MES, 0.8-1.2 M sodium chloride, 8-16% PEG3350 |