5HDQ
MntC co-structure with mAB 305-78-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.712, 41.733, 108.404 |
| Unit cell angles | 90.00, 97.09, 90.00 |
Refinement procedure
| Resolution | 36.600 - 1.830 |
| R-factor | 0.2086 |
| Rwork | 0.207 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.9.7) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.600 |
| High resolution limit [Å] | 1.830 |
| Number of reflections | 43860 |
| <I/σ(I)> | 1 |
| Completeness [%] | 61.6 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Protein complex (10 mM HEPES pH 7.5 and 150 mM NaCl) was concentrated to 5.16 mg/mL and crystals grown by the hanging drop method using 1 M LiCl, 0.1 M MES, pH 6.0 and 20% PEG 6K as the precipitating reagents. |
