5HDO

Crystal structure of a nanobody raised against urokinase-type plasminogen activator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-07
Detector	PSI PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	99.830, 99.830, 127.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.277 - 2.160
R-factor	0.1786
Rwork	0.177
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jvp
RMSD bond length	0.007
RMSD bond angle	1.093
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.280	2.237
High resolution limit [Å]	2.160	2.160
Rmerge	0.085	0.690
Number of reflections	35146
<I/σ(I)>	22.42	4.18
Completeness [%]	99.9	99.88
Redundancy	14.5	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.1M MES, 0.2M ammonium sulphate, 30% w/v PEG 5000