5HBZ

Structure of EAV NSP11 K170A mutant at 3.10A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	248.268, 248.268, 226.298
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.100
R-factor	0.19508
Rwork	0.195
R-free	0.19893
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eyi
RMSD bond length	0.009
RMSD bond angle	1.438
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.150
High resolution limit [Å]	3.100	3.100
Number of reflections	44766
<I/σ(I)>	16
Completeness [%]	99.2
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	PEG