5HBX
RNA primer-template complex with 2-methylimidazole-activated monomer analogue-2 binding sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 3 |
| Unit cell lengths | 43.545, 43.545, 83.996 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.21478 |
| Rwork | 0.213 |
| R-free | 0.24899 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dhc |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.770 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.128 | 0.488 |
| Number of reflections | 19566 | |
| <I/σ(I)> | 14.6 | 2.64 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | Ammonium acetate, Magnesium chloride hexahydrate,0.05 M TRIS hydrochloride pH 7.5,10% v/v (+/-)-2-Methyl-2,4-pentanediol |
