5HBD
Filamentous Assembly of Green Fluorescent Protein Supported by a C-terminal fusion of 18-residues, viewed in space group C2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.700, 51.340, 55.060 |
Unit cell angles | 90.00, 99.64, 90.00 |
Refinement procedure
Resolution | 55.620 - 1.650 |
R-factor | 0.17628 |
Rwork | 0.173 |
R-free | 0.20475 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.696 |
Data scaling software | XSCALE (Oct 15, 2015) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.620 | 1.750 | |
High resolution limit [Å] | 1.650 | 4.920 | 1.650 |
Rmerge | 0.094 | 0.036 | 0.534 |
Rmeas | 0.103 | 0.040 | 0.645 |
Total number of observations | 205218 | ||
Number of reflections | 34226 | 1463 | 3851 |
<I/σ(I)> | 13.65 | 35.31 | 2.29 |
Completeness [%] | 91.2 | 98.4 | 63.8 |
Redundancy | 6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M sodium citrate, 10% MPD, 0.1M HEPES, pH 7.5 |