5HB8

Crystal structure of Chaetomium thermophilum Nup53 RRM (space group P3121)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 31 2 1
Unit cell lengths	94.660, 94.660, 115.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.330 - 1.700
R-factor	0.1643
Rwork	0.163
R-free	0.21010
RMSD bond length	0.010
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.700
Number of reflections	60735
<I/σ(I)>	37.3
Completeness [%]	91.1
Redundancy	35.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	4 % Tacsimate (pH 4.1), 15 % (w/v) PEG 3350