5HB3

Crystal structure of Chaetomium thermophilum Nic96 SOL-Nup53 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	59.520, 86.950, 98.010
Unit cell angles	100.69, 99.59, 95.66

Refinement procedure

Resolution	47.285 - 2.650
R-factor	0.2134
Rwork	0.212
R-free	0.24880
RMSD bond length	0.002
RMSD bond angle	0.562
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.650
Number of reflections	48566
<I/σ(I)>	15.1
Completeness [%]	88.6
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	0.1 M TRIS (pH 7.0), 12 % (w/v) PEG 6000, 1.2 M sodium chloride