5HAY

Crystal structure of Chaetomium thermophilum Nup170 CTD Y905M L1007M L1183M V1292M mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-01-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	61.790, 101.700, 193.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.185 - 2.800
R-factor	0.2113
Rwork	0.209
R-free	0.25070
RMSD bond length	0.003
RMSD bond angle	0.555
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	30966
<I/σ(I)>	22.4
Completeness [%]	98.2
Redundancy	16.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	294	0.1 M MES (pH 6.3), 10 % PEG 20000, 10 % (v/v) ethylene glycol, 0.2 M potassium thiocyanate