5H9U

Crystal structure of a thermostable methionine adenosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	230.024, 77.053, 96.792
Unit cell angles	90.00, 112.04, 90.00

Refinement procedure

Resolution	43.240 - 2.667
R-factor	0.1759
Rwork	0.173
R-free	0.23270
RMSD bond length	0.006
RMSD bond angle	1.256
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.240
High resolution limit [Å]	2.667
Number of reflections	44867
<I/σ(I)>	11.4
Completeness [%]	100.0
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.01M Iron chloride hexahydrate, 0.1M Sodium citrate tribasic dehydrate pH 5.6, 10% Jeffamine M-600