5H9S

Crystal Structure of Human Galectin-7 in Complex with TAZTDG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	53.873, 64.475, 73.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.663 - 1.821
R-factor	0.1867
Rwork	0.183
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkz
RMSD bond length	0.008
RMSD bond angle	1.142
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.890
High resolution limit [Å]	1.820	1.820
Rmerge	0.033	0.406
Number of reflections	23368
<I/σ(I)>	44.962	3.95
Completeness [%]	99.8	100
Redundancy	6.9	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.22 M MgAcetate, 21% (w/v) PEG 3350