5H8O

Crystal structure of an ASC-binding nanobody in complex with the CARD domain of ASC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979100
Spacegroup name	P 1 2 1
Unit cell lengths	53.625, 30.996, 79.244
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.240 - 4.206
R-factor	0.303
Rwork	0.300
R-free	0.35560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.151
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.240	79.240	4.430
High resolution limit [Å]	4.200	13.280	4.200
Rmerge	0.824	0.313	1.158
Rpim	0.349	0.139	0.477
Total number of observations	12955	463	1967
Number of reflections	2025
<I/σ(I)>	2.1	3.2	2
Completeness [%]	99.0	99.2	100
Redundancy	6.4	5.9	6.7
CC(1/2)	0.736	0.913	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	291	0.1 M citric acid at pH 3.5 and 3 M sodium chloride