5H84
Human Gcn5 bound to propionyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-22 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 |
| Unit cell lengths | 38.087, 38.087, 187.057 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.170 - 2.000 |
| R-factor | 0.1652 |
| Rwork | 0.164 |
| R-free | 0.19534 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z4r |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.170 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.087 | 0.313 |
| Number of reflections | 10102 | |
| <I/σ(I)> | 13.91 | 5.68 |
| Completeness [%] | 98.0 | 92.71 |
| Redundancy | 5.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 20% (v/v) ethanol, 100 mM Tris, pH 9.0 |
