5H80
Biotin Carboxylase domain of single-chain bacterial carboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 138.100, 138.100, 97.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.740 - 1.700 |
| R-factor | 0.15084 |
| Rwork | 0.149 |
| R-free | 0.18441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dzd |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.371 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.740 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.095 | |
| Number of reflections | 116236 | |
| <I/σ(I)> | 15.61 | 1.18 |
| Completeness [%] | 99.9 | 97.8 |
| Redundancy | 10.1 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | MgCl2 HEPES (pH 7.5) poly(acrylic acid sodium salt) 5,100. |
