5H7Y
Structure of immunity protein TplEi of T6SS from Pseudomonas aeruginosa complexed with "L" peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97876 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 113.614, 113.614, 128.131 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.798 - 2.195 |
| R-factor | 0.1839 |
| Rwork | 0.182 |
| R-free | 0.22190 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.188 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.130 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.583 |
| Number of reflections | 25539 | |
| <I/σ(I)> | 26.69 | 5.51 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 16.22 | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 0.1 M Sodium HEPES, 20%(w/v) PEG 10000 |
