5H7W

Crystal structure of 5'-nucleotidase from venom of Naja atra

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	53.089, 68.149, 139.815
Unit cell angles	90.00, 95.73, 90.00

Refinement procedure

Resolution	24.980 - 1.900
R-factor	0.157
Rwork	0.155
R-free	0.19700
RMSD bond length	0.008
RMSD bond angle	1.136
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.076	0.754
Number of reflections	88970
<I/σ(I)>	22.9	2.6
Completeness [%]	93.0	89.4
Redundancy	6.5	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2M calcium acetate, 20% PEG 3350