5H7N
Crystal structure of human NLRP12-PYD with a MBP tag
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.540, 104.550, 110.940 |
Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
Resolution | 47.182 - 1.849 |
R-factor | 0.1806 |
Rwork | 0.179 |
R-free | 0.22370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ifp |
RMSD bond length | 0.014 |
RMSD bond angle | 1.310 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 161270 | |
<I/σ(I)> | 17.3 | 1.7 |
Completeness [%] | 98.6 | 96 |
Redundancy | 5.7 | 5.6 |
CC(1/2) | 0.998 | 0.636 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 30% PEG 550, 0.2M Ammonium Sulfate, NaAc 4.6 |