5H79
Crystal structure of a repeat protein with three Protein A repeat module
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-04-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.689, 46.614, 74.311 |
Unit cell angles | 90.00, 107.29, 90.00 |
Refinement procedure
Resolution | 28.018 - 2.700 |
R-factor | 0.2321 |
Rwork | 0.230 |
R-free | 0.27780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4znc 5coc |
RMSD bond length | 0.003 |
RMSD bond angle | 0.630 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 10513 | |
<I/σ(I)> | 15.5 | 2.14 |
Completeness [%] | 98.7 | 98.6 |
Redundancy | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.5 | 296 | 0.1M sodium acetate pH 4.5, 13.5%(w/v) PEG 1000 |