5H77

Crystal structure of the PKA-protein A fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	80
Detector technology	CCD
Collection date	2016-03-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	71.588, 135.378, 88.988
Unit cell angles	90.00, 108.77, 90.00

Refinement procedure

Resolution	42.419 - 3.197
R-factor	0.2424
Rwork	0.239
R-free	0.28450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2izx 2spz
RMSD bond length	0.005
RMSD bond angle	0.990
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	26459
<I/σ(I)>	11.2	1.8
Completeness [%]	99.4	99.1
Redundancy	3.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	296	0.1M sodium acetate pH 4.5, 0.68M sodium citrate pH 4.5