5H75

Crystal structure of the MrsD-Protein A fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	80
Detector technology	CCD
Collection date	2015-12-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 21 3
Unit cell lengths	167.729, 167.729, 167.729
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.623 - 2.738
R-factor	0.2059
Rwork	0.204
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p3y 2spz
RMSD bond length	0.006
RMSD bond angle	0.877
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	39652
<I/σ(I)>	22.1
Completeness [%]	92.0
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	277	0.94M sodium citrate pH 5.5