5H71
Structure of alginate-binding protein AlgQ2 in complex with an alginate trisaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-09 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.274, 53.150, 127.451 |
| Unit cell angles | 90.00, 93.63, 90.00 |
Refinement procedure
| Resolution | 29.852 - 1.549 |
| R-factor | 0.1686 |
| Rwork | 0.167 |
| R-free | 0.19650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j1n |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.126 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.580 |
| High resolution limit [Å] | 1.549 | 1.549 |
| Rmerge | 0.084 | 0.420 |
| Number of reflections | 143400 | |
| <I/σ(I)> | 25.9 | 3.2 |
| Completeness [%] | 96.8 | 89.1 |
| Redundancy | 3.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 30% PEG4000, 0.2 M lithium sulfate, 0.1 M tris(hydroxymethyl)aminomethan-hydrochloride acid |
