5H6T
Crystal structure of Hydrazidase from Microbacterium sp. strain HM58-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2012-11-11 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 122.162, 128.068, 159.304 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.970 - 1.600 |
R-factor | 0.16551 |
Rwork | 0.165 |
R-free | 0.17809 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h0l |
RMSD bond length | 0.008 |
RMSD bond angle | 1.390 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MoRDa |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.054 | 0.232 |
Number of reflections | 163385 | |
<I/σ(I)> | 47.2 | 8.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.1 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M imidazole, 0.17 M LiSO4, 2 M (NH4)2SO4 |