5H5A
Mdm12 from K. lactis (1-239), Lys residues are uniformly dimethyl modified
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.868, 48.524, 130.126 |
| Unit cell angles | 90.01, 91.50, 90.01 |
Refinement procedure
| Resolution | 50.000 - 2.260 |
| R-factor | 0.25248 |
| Rwork | 0.251 |
| R-free | 0.28615 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.003 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 527803 |
| <I/σ(I)> | 11.3 |
| Completeness [%] | 97.1 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.26M NaH2PO4, 0.14M K2HPO4 pH 5.6 |
