5H58
Structural and dynamics studies of the TetR family protein, CprB from Streptomyces coelicolor in complex with its biological operator sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9737 |
| Spacegroup name | P 32 |
| Unit cell lengths | 149.810, 149.810, 70.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.249 - 3.991 |
| R-factor | 0.2215 |
| Rwork | 0.217 |
| R-free | 0.28160 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.559 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.950 |
| Number of reflections | 15346 |
| <I/σ(I)> | 22.4 |
| Completeness [%] | 99.7 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.1 M ammonium acetate, 0.02 M MgCl2.6H2O, 0.05 M HEPES sodium, 5% PEG 8000 |
