5H4Z
Crystal structure of S202G mutant of human SYT-5 C2A domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97916 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 76.005, 118.656, 109.464 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.010 |
R-factor | 0.20254 |
Rwork | 0.200 |
R-free | 0.25745 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h4y |
RMSD bond length | 0.011 |
RMSD bond angle | 1.331 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.167 | 0.837 |
Number of reflections | 9990 | |
<I/σ(I)> | 15.6 | 3.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 287 | 0.1M calcium acetate, 0.1M sodium acetate pH 4.0, 10%(w/v) PEG4000 |