5H43

Structural and mechanistical studies of the nuclear import by Importin-alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0050
Spacegroup name	P 41 21 2
Unit cell lengths	82.222, 82.222, 169.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.900 - 2.300
R-factor	0.21298
Rwork	0.211
R-free	0.24253
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3knd
RMSD bond length	0.016
RMSD bond angle	1.805
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.370
High resolution limit [Å]	2.300	2.310
Rmerge	0.090
Number of reflections	34657
<I/σ(I)>	34	5.7
Completeness [%]	99.9	100
Redundancy	18.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	3%(v/v) Tacsimate pH5.0, 0.1M Sodium citrate tribasic dehydrate pH5.6, 16%(w/v) PEG 3350