5H41
Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with sophorose, isofagomine, sulfate ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-28 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.692, 94.877, 157.357 |
Unit cell angles | 90.00, 100.81, 90.00 |
Refinement procedure
Resolution | 48.270 - 2.000 |
R-factor | 0.16954 |
Rwork | 0.168 |
R-free | 0.20406 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h3z |
RMSD bond length | 0.017 |
RMSD bond angle | 1.704 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.127 | 0.502 |
Number of reflections | 168440 | |
<I/σ(I)> | 3.7 | 1.6 |
Completeness [%] | 96.0 | 99 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG3350, calcium acetate, Tris-HCl |