5H3X

The structure of the N-terminal of the fibronectin/fibrinogen-binding protein from Streptococcus suis (FBPS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 1W2B
Synchrotron site	BSRF
Beamline	1W2B
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-03
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	70.555, 70.555, 110.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.597 - 2.100
R-factor	0.2175
Rwork	0.216
R-free	0.24550
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.215
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.084	0.512
Number of reflections	16622
<I/σ(I)>	29.98
Completeness [%]	97.6
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Na Citrate (pH 5.6), 20%(w/v) PEG 4,000, 0.2M NaCl