5H3T
m7G cap bound to GEMIN5-WD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 113.159, 91.007, 153.717 |
| Unit cell angles | 90.00, 100.59, 90.00 |
Refinement procedure
| Resolution | 49.326 - 2.571 |
| R-factor | 0.1738 |
| Rwork | 0.171 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ymu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.109 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2386: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 2.610 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.070 | 0.734 |
| Number of reflections | 96204 | |
| <I/σ(I)> | 16.4 | 2 |
| Completeness [%] | 97.9 | 71.1 |
| Redundancy | 7 | 5.5 |
| CC(1/2) | 0.990 | 0.722 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.1 M Bis-Tris propane, 0.2 M Na/K phosphate, 20% PEG 3350 |
