5H3S
apo form of GEMIN5-WD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.503, 91.373, 112.571 |
| Unit cell angles | 90.00, 100.55, 90.00 |
Refinement procedure
| Resolution | 29.366 - 3.000 |
| R-factor | 0.2037 |
| Rwork | 0.201 |
| R-free | 0.26060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ymu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.688 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2386: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.370 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.112 | |
| Number of reflections | 30421 | |
| <I/σ(I)> | 11.4 | 2.2 |
| Completeness [%] | 99.0 | 99.6 |
| Redundancy | 5.8 | 6 |
| CC(1/2) | 0.997 | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 288 | 0.1 M Na/K phosphate, 20% PEG 3350 |
