5H39
Structural analysis of KSHV thymidylate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-13 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.813, 78.841, 67.642 |
| Unit cell angles | 90.00, 116.03, 90.00 |
Refinement procedure
| Resolution | 19.878 - 2.000 |
| R-factor | 0.1627 |
| Rwork | 0.161 |
| R-free | 0.20150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h38 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.160 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.994 | 2.000 |
| Number of reflections | 40983 | |
| <I/σ(I)> | 19.3 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 300 | potassium phosphate (monobasic), PEG 8K, glycerol |
