5H2G

Crystal structure of oxidized DapF from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-20
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	I 2 2 2
Unit cell lengths	101.738, 119.079, 155.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.560 - 2.000
R-factor	0.1902
Rwork	0.189
R-free	0.21910
Structure solution method	SAD
RMSD bond length	0.023
RMSD bond angle	2.137
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.560	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	59911
<I/σ(I)>	5.8	5.1
Completeness [%]	98.7	100
Redundancy	8.1	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	Ammonium sulfate, Bis-Tris