5H29
Crystal Structure of the NTD_N/C domain of Alkylhydroperoxide Reductase AhpF from Enterococcus Faecalis (V583)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 134.278, 134.278, 134.278 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.320 - 2.300 |
| R-factor | 0.19558 |
| Rwork | 0.194 |
| R-free | 0.22871 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zyn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.947 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.051 | 0.660 |
| Number of reflections | 19025 | |
| <I/σ(I)> | 22.1 | 3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.4 | 11.7 |
| CC(1/2) | 0.990 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M Bis-Tris (pH 6.5), 2.0 M ammonium sulfate and 0.1 M sodium chloride |
