5H1X
Crystal Structure of rat Nup62 Coiled-coil motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-16 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.982, 97.563, 31.502 |
Unit cell angles | 90.00, 112.85, 90.00 |
Refinement procedure
Resolution | 27.825 - 2.410 |
R-factor | 0.2351 |
Rwork | 0.227 |
R-free | 0.30770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t97 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.120 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX (1.8.4_1496) |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.782 | 48.782 | 2.540 |
High resolution limit [Å] | 2.408 | 7.620 | 2.410 |
Rmerge | 0.026 | 0.176 | |
Rmeas | 0.047 | 0.032 | 0.211 |
Rpim | 0.025 | 0.017 | 0.114 |
Total number of observations | 22313 | 744 | 3034 |
Number of reflections | 6669 | ||
<I/σ(I)> | 17.9 | 38.5 | 5.9 |
Completeness [%] | 99.4 | 98.9 | 98.6 |
Redundancy | 3.3 | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | 291 | 100mM Tris-HCl, 50mM Magnesium Chloride, 34% PEG 400 |