5H19

EED in complex with PRC2 allosteric inhibitor EED162

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979112
Spacegroup name	C 2 2 21
Unit cell lengths	50.490, 90.990, 179.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.190 - 1.900
R-factor	0.1786
Rwork	0.177
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qxv
RMSD bond length	0.010
RMSD bond angle	1.050
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.190	1.911
High resolution limit [Å]	1.900	1.905
Rmerge	0.134
Number of reflections	33040
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M Bis-Tris, 0.2 M MgCl2, 20% PEG 3350