5H10

TRAF1-TANk complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	110
Detector technology	CCD
Collection date	2016-06-01
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	77.210, 79.034, 113.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.890 - 3.205
R-factor	0.2235
Rwork	0.218
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e1t
RMSD bond length	0.002
RMSD bond angle	0.517
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.250
High resolution limit [Å]	3.200	3.200
Rmerge	0.065	0.172
Number of reflections	11811
<I/σ(I)>	42.34
Completeness [%]	99.5
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.38M Ammonium sulfate, 0.1M CHES pH 9.3, 200mM NaCl