5H0V

Crystal structure of H88A mutated human transthyretin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	64.710, 84.660, 85.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.490 - 1.580
R-factor	0.1803
Rwork	0.179
R-free	0.20887
RMSD bond length	0.014
RMSD bond angle	1.689
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.500
High resolution limit [Å]	1.580
Rmerge	0.089
Number of reflections	64837
<I/σ(I)>	14.6
Completeness [%]	100.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	16% (+/-)-2-methyl-2,4-pentanediol, 0.2 M MgCl2, 0.1 M sodium acetate