5H0U
Crystal structure of the catalytic domain of membrane type 1 matrix metalloproteinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-02 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.995, 62.995, 122.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.544 - 2.239 |
| R-factor | 0.1982 |
| Rwork | 0.195 |
| R-free | 0.25380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bqq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.008 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2140: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.544 | 2.370 |
| High resolution limit [Å] | 2.239 | 2.239 |
| Rmerge | 0.105 | 0.998 |
| Number of reflections | 12491 | |
| <I/σ(I)> | 16.8 | 2.3 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | Ammonium nitrate, PEG3350 |
